Building a Biofilm Model from a Confocal Microscope Stack

1 Contents

1 Introduction to Biofilms

2 Biofilm Growth and Development

3 Beneficial and Naturally Occurring Biofilms

4 Biofilms in Health and Medicine

5 Biofilms in Industrial Environments

6 Characterizing and Understanding Biofilms

7 Controlling Biofilms

8 Methods for Studying Biofilms

9 Mathematical Models of Biofilms

10 Diffusion in Biofilms

11 Lab Exercises

        Overview and Instructions

        Building and Using a Batch Biofilm Growth Reactor (Mason Jar Reactor)

        Building a Biofilm Model from a Confocal Microscope Stack

        Building and Using a Biofilm Continuous Flow Stirred Reactor

        Building and Using a Flow Cell Biofilm Reactor

        Collecting Soil Biofilms by the Buried Slide Technique

        Colorimetric Measurement of Biofilm Density

        Drop Plate Method for Counting Biofilm Cells

        Flow Through Gram Stain

        Harvesting and Dispersing of Cells from Biofilms (Standard Method)

        Harvesting and Dispersing of Cells from Biofilms (Alternative Method)

        Henrici's Microbial Fishing Technique

        Measurement of Biofilm Thickness

        Model System for Growing a Standard Biofilm: Static Glass Coupon Reactor

        Biofilms as Biobarriers

        Dental Biofilms: Detection and Quantification of Plaque

        Isolation of Biofilm Populations from Soil Crumbs by Flotation

        Bacterial Infection in a Cystic Fibrosis Patient

        Buccal Epithelial Cells with Adherent Bacterial Cells Revealed by Negative Staining

        Construction of a Winogradsky Column

        Constructing and Using a Drip Flow Reactor

        Coaggregation: Interactions between Bacteria in Dental Biofilms

        Antimicrobial Sensitivity: Biofilm vs. Planktonic Cells

        Detection of Naturally Occurring Acyl- Homoserine Lactones Using a Chromobacterium Reporter Strain

1 Instructor

2 Student

1 Aids for Instructors

2 References

1 Read Me First

2 System Requirements

3 Navigating